(Javier Gutierrez Jimenez, Sciences and Arts University of Chiapas, Mexico)įigure 14: Bacillus sp._Endospore stain (Enlarged view). stained with Sheaffer-Fulton technique showing green endospores. Finazzo, Broward Community College–Central campus, Davie, FL)įigure 14: Bacillus sp._Endospore stain (Labeled view). Due to the age of the culture, endospores have been released from the cells.

cereus are in red endospores are in green. Endospore stain of a Bacillus cereus culture using the Shaeffer-Fulton method and viewed at 1,000x total magnification under an oil immersion lens. (Brian Kimble, University of Maryland, College Park, MD)įigure 13: Bacillus cereus_Endospore stain (Enlarged view). Bacillus subtilis, an endospore-producing bacterium.

Smith, University of Maryland, College Park, MD)įigure 12: Bacillus subtilis_Endospore stain (Enlarged view). Phase-contrast image of endospore-positive Bacillus species. Smith, University of Maryland, College Park, MD)įigure 11: Bacillus sp._Endospore stain (Enlarged view). Smith, University of Maryland, College Park, MD)įigure 10: Bacillus sp._Endospore stain (Enlarged view). Smith, University of Maryland, College Park, MD)įigure 9: Bacillus sp._Endospore stain (Enlarged view). Endospore-negative Staphylococcus species. Smith, University of Maryland, College Park, MD)įigure 8: Staphylococcus sp._Endospore stain (Enlarged view ). Smith, University of Maryland, College Park, MD)įigure 7: Staphylococcus sp._Endospore stain (Enlarged view). Smith, University of Maryland, College Park, MD)įigure 6: Bacillus subtilis_Endospore stain (Enlarged view). Smith, University of Maryland, College Park, MD)įigure 5: Bacillus sp._Endospore stain (Enlarged view). Smith, University of Maryland, College Park, MD)įigure 4: Bacillus sp._Endospore stain (Enlarged view). Smith, University of Maryland, College Park, MD)įigure 3: Bacillus sp._Endospore stain (Enlarged view). Spore stain(Jerry Keplinger, East Tennessee State University, Johnson City, TN)įigure 2: Bacillus sp._Endospore stain (Enlarged view).

techmicrobio.Download the PowerPoint PowerPoint Contentsįigure 1: Spore stain_Endospore stain (Enlarged view).

Staining procedures - UK Standards for Microbiology Investigations.

A Oktari - The Bacterial Endospore Stain on Schaefferįulton using Variation of Methylene Blue Solution.

Young cultures (less than or a day old) may have only vegetative cells, whereas older cultures (5 to 7 days old) are excellent for good sporulation.ģ- Heat fixing should be done with minimal flaming as excess heat will destroy the integrity of the cells, causing them to shrink and to aggregate together on the slide.Ĥ- It should be noted that any debris on the slide can also take up and hold the malachite green stain and so caution should be taken when interpreting slides.

The smear is therefore ready to be observed under the microscope at the x100 objective with immersion oil.ġ- Some labs do not use paper towels as described in the Schaefer-Fulton procedure (an ill-fitting piece of paper may burn or leak)Ģ- The age of the culture will affect sporulation. Then cover the slide with a mercurochrome solution for two minutes. Wash it with water, wipe it with filter paper. tetani, Bacillus anthracis, Bacillus cereus, Desulfotomaculum spp, Sporolactobacillus spp, Sporosarcina spp,Ĭover the smear with malachite green for a minimum of 45 min. 🏾 Examples of positive endospore staining : Clostridium perfringens, C. 🏾 Vegetative cells : Vegetative cells are brownish-red to pink. Endospore staining (source : Endospores : Endospores are light green.

Bacillus subtilis spore stain